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Features & Benefits

• Stabilized 5X concentrated master mix simplifies reaction assembly and improves accuracy by minimizing pipetting steps
• Optimized, double pre-primed SuperMix provides equal representation of 5′ and 3′ RNA sequences ≤ 1kb for quantitative and conventional two-step RT-PCR
• Improved RNA template binding affinity for increased yields with problematic sequences or complex secondary structure
• Perform more qPCR assays from limiting amounts of RNA with 2-3 fold improved cDNA yields over older enzyme technologies
• Broad linear dynamic detection range spanning up to 108 fold

Description

qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase mutant with reduced RNase H activity and improved yield and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than our previous qScript cDNA SuperMix cDNA synthesis kits, which utilize an engineered RNase H(+) reverse transcriptase mutant.

Details

Contents

Single-tube, 5X concentrated reagent containing:

• Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
• Recombinant ribonuclease inhibitor protein (RIP)
• qScript XLT reverse transcriptase
• Titrated concentrations of random hexamer and oligo(dT) primer
• Proprietary enzyme stabilizers and performance-enhancing additives