biotechrabbit™ CAPITAL qPCR Probe Master Mix for quantifying genomic, cDNA and viral sequences provides outstanding performance in single and multiplex qPCR. The high sensitivity provided by the mix is ideal for detection of low-abundance DNA targets in various applications, such as pathogen detection.
CAPITAL qPCR Probe Master Mix uses proprietary combination of enzyme and buffer chemistry for efficient extension and early Ct in single and multiplex qPCR. To enable the use of the kit on qPCR platforms with different reference dye concentration requirements, three kit formats are available: a kit containing no ROX, as well as LRox and HRox versions containing ROX in the corresponding concentrations.
Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).