biotechrabbit RTS pIVEX Wheat Germ His6-tag Vector Set

• Stable circular templates ensure high yields in preparative-scale expression reactions
• For construction of C- and N-terminal His6-tag proteins

The RTS pIVEX Wheat Germ vectors are designed for high yield cell-free expression of His6-tagged eukaryotic proteins using the RTS Wheat Germ kits.

The two vectors contain all regulatory elements necessary for in vitro expression based on a combination of T7 RNA polymerase and wheat germ lysates. They enable the attachment of a His6-tag in different orientations, at the C- and N-terminus of the protein. Cloning into RTS pIVEX Wheat Germ His6-tag vectors allows optimal protein expression in RTS Wheat Germ CECF kits to produce more than 1 mg protein per 1 ml synthesis volume in a 24-hour reaction.

(former product codes: RiNA N-3150-02, 5PRIME 2402010)

• Standard or hot-start PCR
• Long-range and high-fidelity PCR
• cDNA synthesis and RT-PCR
• qPCR
• Sequencing
• DNA labeling

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye