General description
Antibiotic Antimycotic Solution is formulated to contain
10,000 units penicillin, 10 mg streptomycin and 25μg Amphotericin B per mL, and
is solubilized in a proprietary citrate buffer. It is recommended for use in
cell culture applications at 10mL/L. Antibioticantimycotic solution is
generally used to prevent the growth of harmful microorganisms including
bacteria and fungi in cell culture media.[1]
Application
Antibiotic antimycotic solution has been used:
- as a
supplement in different types of cell culture media.[2][3][4]
- as a
component of phosphate buffer saline (PBS) sterile solution to prevent
contamination of periodontal ligament fibroblasts (PDLF) tissues that are
isolated and cultured in the experiments.[5]
- as a
component of storage solution for preserving tracheal segments harvested
for subsequent experimental investigation.[6]
Biochem/physiol Actions
Mode of Action: Penicillin acts by inhibiting bacterial
cell-wall synthesis. Streptomycin inhibits prokaryote protein synthesis by
preventing the transition from initiation complex to chain-elongating ribosome
and causes miscoding. Amphotericin B interferes with fungal membrane
permeability by forming channels in the membranes and causing small molecules
to leak out.
Antimicrobial spectrum: Includes Gram-negative bacteria, Gram-positive
bacteria, fungi and yeasts.
