Product Name:

Purospher® STAR NH2 (5 µm), L × I.D. 250 mm x 4 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use

Material:  stainless steel column

Quality Level:  100

Agency:  suitable for USP L8

product line:  Purospher®

feature:  endcapped: no

packaging:  1 ea of

extent of labeling:  3.5% Carbon loading

parameter:

250 bar pressure

60 °C max. temp.

technique(s):

HPLC: suitable

LC/MS: suitable

mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.:  25 cm × 4 mm

surface area:  330 m2/g

matrix:  fully porous particle

matrix active group:  NH2 bonding phase

particle size:  5 μm

pore size:  120 Å pore size

operating pH range:  2-7.5

separation technique:

hydrophilic interaction (HILIC)

normal phase

reversed phase

storage temp.:  no temp limit

General description

Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phaseconsisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm LiChroCART™ 250-4 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART™ 4-4 part number 1.50267.0001. LiChroCART™ cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.

Features and Benefits

• Ultra-pure silica (99.999%)
• Low silanol activity
• Reproducible results from run-to-run and batch-to-batch

Analysis Note

Pressure: ≤ 60 bar

Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000

Symmetry (2-Nitrotoluene): 0.8 - 1.4

Capacity factor (2-Nitrotoluene): 0.5 – 1.8

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany

MANUCART is a trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany