Abcam's Phosphate Assay Kit (Fluorometric) provides a sensitive, easy, quick means of assessing phosphate over a
wide range of concentrations. In the assay, inorganic phosphate reacted with maltose to produce glucose in the
present of a special enzyme. The glucose is specifically oxidized to generate a product that reacts with the OxiRed
probe to generate fluorescence (Ex/Em=535/587nm). The kit can be used to detect Pi produced through reactions
involving ATPases, GTPases, 5'-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and
phosphorylase, etc. from a variety of samples. Glucose background can be eliminated by doing a glucose control.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K420 Phosphate Assay Kit
(Fluorometric). K420-100 is the same size as the 100 test size of ab102508.
Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One
of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the
various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in
variety samples or monitor Pi changes during kinase and phosphatase reactions.
