abcam Fumarate Assay Kit

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C  

Appropriate long-term storage conditions

-20°C  

Storage information

-20°C  

Fumarate Assay Kit ab102516 provides a convenient tool for sensitive detection of Fumarate in biological samples.

In the fumarate assay protocol, the fumarate enzyme mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (λ = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 μM).

Fumurate assay protocol summary:

- add samples and standards to wells

- add reaction mix and incubate for 1 hr

- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K633 Fumarate Colorimetric Assay Kit. K633-100 is the same size as the 100 test size of ab102516.

Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb's cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.

It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye