Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Xanthine Oxidase Activity Assay Kit ab102522 is an easy and
sensitive assay to determine xanthine oxidase activity in variety of samples.
In the xanthine oxidase assay protocol, xanthine oxidase
oxidizes xanthine to hydrogen peroxide (H2O2) which
reacts stoichiometrically with a probe to generate color (at OD = 570 nm) and
fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity
is proportional to XO content, the XO activity can be accurately measured.
The kit detects 1-100 mU xanthine oxidase in 100 μl reaction
volume.
Xanthine oxidase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader immediately and after 10-20 min
This product is manufactured by BioVision, an Abcam company
and was previously called K710 Xanthine Oxidase Activity
Colorimetric/Fluorometric Assay Kit. K710-100 is the same size as the 100 test
size of ab102522.
Xanthine oxidase (XO, EC 1.17.3.2 ) is present in
appreciable amounts in the liver and jejunum in healthy individuals. However,
in various liver disorders, XO is released into circulation. Therefore,
determination of serum XO level serves as a sensitive indicator of acute liver
damage such as jaundice.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.