|
Abcam's Alcohol Dehydrogenase Assay Kit provides a
convenient tool for sensitive detection of the Alcohol DH in a variety of
samples. In the assay Alcohol DH will utilize isopropanol as a substrate
leading to a proportional color development. The activity of ADH can be easily
quantified colorimetrically (? = 450 nm). This assay detects ADH activity as
low as 0.01 mU in samples.
Visit our FAQs
page for tips and troubleshooting. Alcohol dehydrogenase assay protocol summary:
- add samples and standards to wells
- add reaction mix - incubate for 3 min
- analyze with microplate reader, incubate for 30 min to 2 hrs and analyze
again This product is manufactured by BioVision, an Abcam company
and was previously called K787 Alcohol Dehydrogenase Activity Colorimetric
Assay Kit. K787-100 is the same size as the 100 test size of ab102533. Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a
group of seven dehydrogenase enzymes that occur in many organisms and
facilitate the interconversion between alcohols and aldehydes or ketones with
the reduction of NAD+ to NADH. In humans and many other animals, they serve to
break down alcohols which could otherwise be toxic; in yeast and many bacteria,
some alcohol dehydrogenases catalyze the opposite reaction as part of
fermentation. The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section. |
