abcam Aspartate Aminotransferase Activity Assay Kit

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

How the assay works

Aspartate Aminotransferase Activity Assay Kit measures Aspartate Aminotransferase activity by detecting the transfer of an amino group from aspartate to α-ketoglutarate, producing glutamate and oxaloacetate. In the AST assay protocol, an amino group is transferred from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that converts a nearly colorless probe to color (λmax = 450 nm).

Aspartate Aminotransferase Activity Assay Kit protocol summary

- Add samples and standards to wells

- Add reaction mix

- Incubate at 37°C and analyze with microplate reader after 10 mins and then again after 60 mins

How other researchers are using

Aspartate Aminotransferase Activity Assay Kit has been used in a variety of sample type including:

Mouse cirrhosis-hepatocellular carcinoma 1

Diabetic rat blood samples 2

Blood sample from obese rats 3

References: 

1-Awad B et al. 2023

2-Umar U et al. 2023

3-Liao J et al. 2023

Related and recommended products

For fluorometric detection, we recommend Aspartate Aminotransferase Activity Assay Kit (Fluorometric) Aspartate Aminotransferase Activity Assay Kit (Fluorometric)ab138878

This product is manufactured by BioVision, an Abcam company and was previously called K753 Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit. K753-100 is the same size as the 100 test size of ab105135.

Aspartate aminotransferase (AST), also known as Aspartate transaminase and Glutamate-oxaloacetate transaminase (GOT), is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye