abcam Asparaginase Activity Assay Kit

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples.

In the asparaginase assay protocol, asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected indirectly colorimetrically (OD=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.

Asparaginase assay protocol summary:

- add reaction mix to sample and standard wells, mix and incubate for 10 min

- analyze with microplate reader in kinetic mode for 30-60 min

This product is manufactured by BioVision, an Abcam company and was previously called K754 Asparaginase Activity Colorimetric/Fluorometric Assay Kit. K754-100 is the same size as the 100 test size of ab107922.

Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs).

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye