abcam Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C  

Appropriate long-term storage conditions

-20°C  

Storage information

-20°C  

Abcam's Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923) is a simple, sensitive and rapid means of quantifying glucose-6-Phosphate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). This assay can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 μM in the original sample assuming a dilution of 5X during processing. This Glucose-6-Phosphate Assay Kit - High Sensitivity is more sensitive than our colorimetric assay, Glucose-6-Phosphate Assay Kit (Glucose-6-Phosphate Assay Kit (Colorimetric)ab83426).

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K687 PicoProbe™ Glucose-6-Phosphate Fluorometric Assay Kit. K687-100 is the same size as the 100 test size of ab107923.

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye