How the assay works
In Formate Assay Kit protocol, formate is oxidized to generate a product resulting in color formation (λ = 450 nm) proportional to formate concentration. The amount of formate in the sample is then determined by comparing the color to a standard curve.
Formate Assay Kit protocol summary:
- Add samples, standards and positive control to wells
- Add formate reagent
- Incubate for 60 minutes at at 37°C.
- Measure Optical Density (OD450 nm) in microplate reader.
This product is manufactured by BioVision, an Abcam company and was previously called K653 Formate Colorimetric Assay Kit. K653-100 is the same size as the 100 test size of ab111748.
Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
