How the assay works
In Neutrophil Elastase Inhibitor Assay Kit protocol, Neutrophil Elastase hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent neutrophil elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.
Neutrophil Elastase Inhibitor Assay Kit protocol summary
- Test inhibitor preparation Neutrophil elastase preparation
- Incubate for 5 mins at 37°C
- Add substrate mix
- Measure fluorescence (Ex/Em = 400/505 nm) in a kinetic mode
- Incubate for 30 mins at 37°C protected from light
- Measure fluorescence (Ex/Em = 400/505 nm) in a kinetic mode
Neutrophil Elastase Inhibitor Assay Kit has been used in a variety of sample type including:
C. mas L. extracts 1
B. officinalis herb extracts 2
Psidium guajava L. extracts 3
References:
1-Zagуrska-Dziok et al. 2023
2-Michalak M al. 2023
3-Bao S et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K782 Neutrophil Elastase Inhibitor Screening Kit (Fluorometric). K782-100 is the same size as the 100 test size of ab118971.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
