In Abcam's MMP-1 Inhibitor Screening Kit, MMP-1 hydrolyzes a specific FRET substrate to release a quenched
fluorescent group, which can be detected at Em/Ex = 490/520nm. In presence of potent MMP-1 inhibitors the
hydrolyzation of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test
suitable as a high throughput screening assay of MMP-1 inhibitors. For comparison of the relative efficacy of test
inhibitors, a control inhibitor, GM 6001 (IC50 = 0.4 nM for MMP-1) is included.
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This product is manufactured by BioVision, an Abcam company and was previously called K794 MMP-1 Inhibitor
Screening Kit (Fluorometric). K794-100 is the same size as the 100 test size of ab118973.
The Matrix metalloproteinase-1 (MMP-1, Interstitial collagenase, fibroblast collagenase) is a member of a multigene
family of calcium-dependent, zinc-containing endoproteinases, the matrix metalloproteinases (MMPs). The MMPs are
responsible for the degradation of the extracellular matrix (ECM) including collagens, elastins, gelatin, matrix
glycoproteins and proteoglycan during normal development and disease processes. MMPs are regulated by
hormones, growth factors and cytokines. MMP-1 belongs to the subclass, the collagenases, and along with MMP-8,
and MMP-13 are the only members of the MMP family that are capable of degrading the types I, II and III interstitial
collagens with high efficiency. These collagens are primarily found in bone, cartilage and skin.
