Protein Carbonyl Content Assay Kit ab126287 is designed to provide a simple and accurate method of quantifying carbonyls in protein samples.
The protein carbonyl assay protocol is based on the reaction of DNPH with protein carbonyls. This is the most popular assay type for measuring protein carbonyl content. DNP hydrazones formed in this reaction are easily quantifiable at 375 nm absorbance.
When used with, for example a 1 mg (~15 nmol) sample, this protein carbonyl assay method has a detection limit of about 0.15 nmol carbonyl. For context, BSA typically contains approximately 1-3 nmol carbonyl/mg.
Protein carbonyl assay protocol summary:
- add DNPH to samples and incubate for 10 min
- add TCA to samples, incubate for 5 min, spin for 2 min, and discard supernatant
- wash pellet by sonicating in acetone, spin and remove acetone, repeat acetone wash step
- add Guanidine solution and resolubilize pellet, and transfer to plate
- analyze with a microplate reader to measure protein carbonyl content, also perform a protein assay (eg. BCA assay) on samples to establish protein concentration
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
