How the assay works
The carbohydrate assay protocol is based on the phenol -
sulfuric acid assay. In the assay, polysaccharides (mono, di, tri, etc.) and
their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers
and converted to furfural or hydroxyfurfural. These react with the developer to
form a chromogen that can be quantified by measuring the absorbance at λ = 490
nm.
Total carbohydrate assay protocol summary:
- Add samples and glucose standards to wells
- Add concentrated sulfuric acid and incubate for 15 min at 90°C
- Add developer and incubate for 5 min
How other researchers are using
Total Carbohydrate Assay Kit - Quantification has been used
in a variety of sample type including:
SHK-1 cell line 1
Blood samples from mice with metabolic syndrome 2
Lattuce seeds extracts 3
References:
1-Martinez D et al. 2022
2-Greco C et al. 2020
3-Rawashdeh Y et al. 2020
Related and recommended products
See an alternative kit to quantify carbohydrates:
High Sensitivity Carbohydrate Assay Kit (Colorimetric) High
Sensitivity Carbohydrate Assay Kit (Colorimetric)ab284543
This product is manufactured by BioVision, an Abcam company
and was previously called K645 Total Carbohydrate Colorimetric Assay Kit.
K645-100 is the same size as the 100 test size of ab155891.
Carbohydrates play important structural as well as chemical
roles in all living systems. Detection of total carbohydrates, therefore, has
wide applications.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
