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How the assay works In ALDH Activity Assay Kit, acetaldehyde is oxidized by ALDH
generating NADH which then reduces a colorless probe to a colored product with
strong absorbance at 450 nm. ALDH assay protocol summary: - Add samples and standards to wells
- Add reaction mix and incubate for 20-60 min
- Analyze with a microplate reader at 5 min and 20-60 min Related and recommended products For fluorometric detection, we recommend ALDH Activity Assay
Kit (Fluorometric) ALDH
Activity Assay Kit (Fluorometric)ab155894 This product is manufactured by BioVision, an Abcam company
and was previously called K731 Aldehyde Dehydrogenase Activity Colorimetric
Assay Kit. K731-100 is the same size as the 100 test size of ab155893. The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a
vital role in cellular detoxification. It oxidizes various aldehydes and
generates the corresponding carboxyolic acid. ALDH have been found in every
cellular compartment. Based on its structure and function, ALDH comprises 3
major classes in mammals: Class 1 and Class 3 (the tumor form) are located in
the cytosol and include both constitutive and induced forms; Class 2 is located
in the mitochondria and only exists as the constitutive form. In humans, the
ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of
function) causes human diseases such as Type II hyperprolinemia,
pyridoxine-dependent seizure and hyperammonemia. Recent studies show that
increased ALDH activity leads to several types of malignancies, serves as a
cancer stem cell marker and correlates with poor prognosis. Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses. |
