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Abcam's 2-Phosphoglycerate Assay kit (ab174097) is a
sensitive, fast and easy-to-use kit. In this assay, 2PG(2-phosphoglycerate) is
converted by Enzyme Mix to PEP (phosphoenolpyruvate), which is further
converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm)
and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence
intensity is proportional to 2PG level. This assay kit can detect 2PG level
below 20 pmol and can be used for a variety of sample types. Visit our FAQs
page for tips and troubleshooting. This product is manufactured by BioVision, an Abcam company
and was previously called K778 2-Phosphoglycerate Colorimetric/Fluorometric
Assay Kit. K778-100 is the same size as the 100 test size of ab174097. 2-phosphoglycerate (2PG) is an important intermediate in the
glycolysis pathway. 2PG is converted by enolase to phosphoenolpyruvate (PEP)
which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is
a highly studied and potentially critical mechanism for ATP generation in
cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a
useful tool for analyzing the glycolytic pathway and its relevance to cancer
research. Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
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