Triglyceride Assay Kit (ab178780) is a sensitive, easy assay
to measure triglyceride concentration in mammalian samples. In this
triglyceride assay protocol, triglycerides are hydrolyzed to glycerol and fatty
acid. The glycerol reacts with Triglyceride Enzyme Mix to form an intermediate
product which in turn reacts with the probe and developer to generate
fluorescence (Ed/Em 535/587 nm). The generated fluorescence is directly
proportional to the amount of triglycerides.
Triglyceride Assay Kit (Fluorometric, Reducing Samples)
ab178780 is suitable for measuring triglyceride levels in samples which contain
reducing substances that may interfere with oxidase-based assays such
as Triglyceride Assay Kit (Triglyceride
Assay Kit - Quantificationab65336).
Triglyceride assay protocol summary:
- add samples and standards to wells
- add lipase and incubate for 20 min
- add reaction mix and incubate for 30 min
- analyze with a microplate reader.
Please note: the general range is 0-10 nmol (colorimetric)
and 0-1 nmol (fluorometric).
Target background
Triglycerides are the main constituent of vegetable oil,
animal fat, LDL and VLDL, and play an important role as transporters of fatty
acids as well as serving as an energy source. Triglycerides are broken down
into fatty acids and glycerol, after which both can serve as substrates for
energy producing and metabolic pathways. High blood levels of triglycerides are
implicated in atherosclerosis, heart disease and stroke as well as in
pancreatitis.
Review our Metabolism Assay Guide to learn about assays for
metabolites, metabolic enzymes, mitochondrial function, and oxidative stress,
and also about how to assay metabolic function in live cells using your plate
reader.
This product is manufactured by BioVision, an Abcam company
and was previously called K614 PicoProbe™ Triglyceride (TG) Fluorometric Assay
Kit. K614-100 is the same size as the 100 test size of ab178780.
How other researchers have used Triglyceride Assay Kit
ab178780
The Triglyceride assay kit has been used in publications in
a variety of sample types, including:
- Human: Hepatocyte cell/cell line extract1,2
- Mouse: Plasma3, Skeletal muscle extract4,
Serum5
References: 1 - Liu Q et al. 2021, PMID:
34341705, 2 - Hu LK et al. 2021, PMID: 34707711, 3 -
Panajatovic MV et al, PMID: 34066911, 4 - Panajatovic MV et al, PMID:
34066911, 5 - Maier JI et al. 2021, PMID: 34203913
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
