Abcam's α-Ketoglutarate Dehydrogenase Activity Assay kit (Colorimetric) (ab185440) provides a quick and easy way
for monitoring α-KGDH activity in various samples. In the assay, α-KGDH converts α-ketoglutarate into an
intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple,
sensitive and can detect α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K678 Alpha-Ketoglutarate
Dehydrogenase Activity Colorimetric Assay Kit. K678-100 is the same size as the 100 test size of ab185440.
α-Ketoglutarate Dehydrogenase (α-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme
complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). α-KGDH
converts α-ketoglutarate into succinylCoA in the presence of NAD and CoA. It is highly regulated by intracellular
ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative
diseases such as Alzheimer's disease. Recent studies show that α-KGDH is a target of oxidative stress; reactive
oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit.
