Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Thrombin Activity Assay Kit (Fluorometric) (ab197006) is
simple, rapid and can detect Thrombin activity as low as 1 ng in samples.
In the thrombin activity assay protocol, a synthetic
AMC-based peptide substrate is proteolytically cleaved by thrombin to release a
fluorophore, AMC, which can be easily quantified by fluorescence reader at
Ex/Em = 350/450 nm.
Thrombin activity assay protocol summary:
- add standards and samples to wells
- add reaction mix
- analyze with a microplate reader for 30-60 min
This product is manufactured by BioVision, an Abcam company
and was previously called K373 Thrombin Activity Fluorometric Assay Kit.
K373-100 is the same size as the 100 test size of ab197006.
Thrombin enzyme (Factor IIa, EC 3.4.21.5), a serine
protease, is an important clotting factor in the coagulation cascade that
involves the conversion of soluble fibrinogen to insoluble active fibrin
strands.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
