Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric)
(ab204695) is an assay where inosine formed from the breakdown of adenosine is
detected via a multi-step reaction, resulting in the formation of an
intermediate that reacts with the ADA Probe to generate a fluorescent product
that can be easily quantified at Ex/Em = 535/587 nm. The kit measures total
activity of Adenosine Deaminase with limit of quantification of 10 μU
recombinant Adenosine Deaminase. The range of the assay is from 10-100 μU.
This product is manufactured by BioVision, an Abcam company
and was previously called K328 Adenosine Deaminase Activity Assay Kit
(Fluorometric). K328-100 is the same size as the 100 test size of ab204695.
Adenosine Deaminase (ADA, EC 3.5.4.4) is an enzyme that
catalyses the conversion of adenosine and 2'-deoxyadenosine to inosine and
2'-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues
and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in
most cells in the body, particularly in lymphocytes and macrophages. It is
present in the cytosol, nucleus and has been found associated with DPP4/CD26 in
the cell membrane. ADA2 was first found in the spleen but is predominantly
found in the plasma and serum. Increased serum ADA levels are found in certain
infectious diseases such as tuberculosis and various liver diseases such as
acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a
few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
