Cyclooxygenase COX Activity Assay Kit (Fluorometric)
(ab204699) provides a simple, sensitive, and high-throughput adaptable method
to detect the peroxidase activity of COX in biological samples or
purified/crude enzyme preparations. The kit includes COX-1 and COX-2 specific
inhibitors to differentiate the activity of COX-1 and COX-2 as well as other
peroxidases, which may be present in the sample. Detection limit: 6 μU/mg.
How the Cox activity assay works
Cyclooxygenase (COX) Activity Assay Kit ab204699 measures
COX peroxidase activity by detecting the conversion of arachidonic acid to
prostaglandin G2 (PGG2), which reacts with a COX probe to produce a fluorescent
signal proportional to enzyme activity.
Cyclooxygenase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- add arachidonic acid/NaOH solution
- analyze with microplate reader every 15 s for 30 min
This product is manufactured by BioVision, an Abcam company
and was previously called K549 Cyclooxygenase (COX) Activity Assay Kit
(Fluorometric). K549-100 is the same size as the 100 test size of ab204699.
Cyclooxygenase (COX), also known as
prostaglandin-endoperoxide synthase (PTGS, EC 1.14.99.1), is an enzyme that is
responsible for the formation of important biological mediators called
prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the
central enzyme in the biosynthetic pathway to prostanoids from arachidonic
acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively
expressed in many tissues and is the predominant form in gastric mucosa and in
kidney. COX-2 is not expressed under normal conditions in most cells, but
elevated levels are observed during inflammation. Pharmacological inhibition of
COX by nonsteroidal anti-inflammatory drugs (NSAID) can provide relief from the
symptoms of inflammation and pain.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
