Dipeptidyl peptidase IV (DPP4) Activity Assay Kit
(Fluorometric) (ab204722) is an assay where DPP4 cleaves a substrate to release
a quenched fluorescent group, AMC (7-Amino-4-Methyl Coumarin), which can be
easily detected at Ex/Em = 360/460 nm. This assay is rapid, simple, sensitive,
and reliable, as well as, suitable for high throughput activity screening of
DPP4. This kit detects DPP4 activity as low as 3 μU per well.
DPP4 assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader for 30 min in kinetic mode
This product is manufactured by BioVision, an Abcam company
and was previously called K779 DPP4 Activity Fluorometric Assay Kit. K779-100
is the same size as the 100 test size of ab204722.
Dipeptidyl peptidase-4 (DPP4), also known as adenosine
deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a
protein that, in humans, is encoded by the DPP4 gene. The substrates of
CD26/DPP4 are proline (or alanine) containing peptides and include growth
factors, chemokines, neuropeptides, and vasoactive peptides. DPP4 plays a major
role in glucose metabolism. It is responsible for the degradation of incretins
such as GLP-1 and hence its inhibition by drugs such as Sitagliptin have been
used for treatment of diabetes mellitus type 2. DPP4 also appears to work as a
suppressor in the development of cancer and tumors.
The standard is affinity purified.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
