Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Tryptophan Assay Kit (Fluorometric) (ab211098) provides a
convenient method to quantify tryptophan present in serum and urine. The assay
can detect bound tryptophan in urine and free and bound tryptophan in serum.
The assay principle is based on a non-enzymatic reaction that uses tryptophan
as a building block, producing an intermediate product that reacts with a
catalyst in order to generate a fluorophore that can be detected at Ex/Em =
370/440 nm.
The reaction is specific for Tryptophan and other amino
acids do not interfere with the assay. The assay can detect as little as 2.5 μM
of tryptophan in a variety of biological samples.
This product is manufactured by BioVision, an Abcam company
and was previously called K557 Tryptophan Assay Kit (Fluorometric). K557-100 is
the same size as the 100 test size of ab211098.
Tryptophan (TRP, W) is one of the eight essential amino
acids, and is mainly used for protein synthesis. Additionally, Tryptophan
serves as a building block for several metabolites including kynurenine,
serotonin, tryptamine, melatonin, niacin, and NAD/NAPD. Tryptophan is the only
amino acid that can be found in blood in two forms: bound (BTRP) and free
(FTRP) Tryptophan. Changes in tryptophan concentrations are directly related to
a number of physiological and behavioral processes including: sleep, memory,
depression, motion sickness, bipolar disorders, and schizophrenia. In general,
tryptophan is the least abundant amino acid in humans. External sources of
tryptophan include chicken, tuna, bananas, cheese, chocolate etc. Chemically,
tryptophan side chain (indole) confers its unique fluorometric properties.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
