How the assay works
In Glycine Assay Kit (Fluorometric), Glycine is oxidized in
the presence of GLY Enzyme Mix is converted to an intermediate, which reacts
with the fluorescent probe to generate a strong stable signal at Ex/Em =
535/587 nm. The intensity of the signal is directly proportional to the amount
of GLY in the sample.
The reaction is specific and other amino acids do not
interfere with the assay.
Glycine Assay Kit (Fluorometric) protocol summary:
- Add samples and standards to wells
- Add reaction mix and incubate for 60 min min
- Analyze with fluorescence microplate reader (Ex/Em = 535/587 nm) in end point
mode
This product is manufactured by BioVision, an Abcam company
and was previously called K589 Glycine Assay Kit (Fluorometric). K589-100 is
the same size as the 100 test size of ab211100.
Glycine (GLY, G) is one of the 20 standard amino acids
commonly found in proteins. Glycine's side chain is a hydrogen substituent,
which makes it the smallest proteogenic and the only non-chiral amino acid.
Basic functions of Glycine include the participation in the synthesis of
creatine, glutathione, heme groups, and conjugated bile acids (bile salts). It
is also present as one of the most abundant residues in the triple-helical
structure of collagen.
Glycine acts as a glucogenic amino acid by regulating sugar
levels in blood. Therefore, glycine supplementation has been used in patients
suffering anemia, hypoglycemia and chronic fatigue. Glycine possesses both
inhibitory and excitatory neurotransmitter functions in the brain stem and
spinal cord. In cancer cells, glycine consumption is highly correlated to
cancer cell proliferation via purine synthesis. Glycine uptake in cancer cell
studies supports the role of this amino acid in tumorigenesis and malignancy.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
