DNase I Assay Kit (Fluorometric) (ab234056) allows for
quantitative evaluation of DNase I activity of purified enzymes and their
inhibitors as well as comparative examination of DNase I activity in biological
samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields
a fluorescent DNA product measured at Ex/Em = 651/681 nm. The limit of
quantification (L.O.Q) is 178 fmoles of DNA probe cleaved per minute per mL.
This product is manufactured by BioVision, an Abcam company
and was previously called K429 DNAse I Activity Assay Kit (Fluorometric).
K429-100 is the same size as the 100 test size of ab234056.
Deoxyribonuclease I (DNase I) is an endonuclease that
cleaves DNA phosphodiester bonds yielding 5′-phosphorylated and 3′-hydroxylated
oligonucleotides. DNase I targets single-stranded DNA, double-stranded DNA, and
chromatin in a non-specific manner. As an important player in cellular waste
management, DNase I is normally secreted extracellularly to clear the system
from circulating cell-free DNA, foreign DNA from food digestion or potential
pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells.
Abnormal DNase I activity occurs in association with a variety of cancers and
auto-immune illnesses that exhibit elevated levels of cell-free DNA.
Furthermore, DNase I has been therapeutically used in cystic fibrosis patients
to degrade DNA and reduce sputum viscosity.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
