abcam FRAP Assay Kit (Ferric Reducing Antioxidant Power Assay)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

FRAP Assay Kit ab234626 provides a quick, sensitive and easy way for measuring the antioxidant capacity of various biological samples. FRAP assay stands for Ferric Reducing Antioxidant Power Assay.

The FRAP assay is high-throughput, adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents.

FRAP assay principle

The assay measures the antioxidant potential in samples through the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the samples. Following the reduction of the ferric iron, a blue color develops that is read colorimetrically at 594 nm.

The antioxidant potential of samples is determined using a ferrous iron standard curve and results are expressed as Fe2+ equivalents (μM) or FRAP value.

FRAP assay protocol summary

- add samples and standards to wells

- add reaction mix

- measure absorbance at 594 nm in kinetic mode for 60 min at 37°C

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye