abcam Nickel Assay Kit

Nickel Assay Kit (ab241008) provides a simple method of quantifying Ni2+ in a variety of samples. The assay takes advantage of reaction of Ni2+ with mercaptoethanol in borate buffer to form a complex with strong absorbance bands from ~300 to 600 nm. Fe2+ and Co2+ interfere with the assay, therefore extra steps (as described below) must be taken to subtract the interference in order to determine the correct Nickel concentration in mixed samples. Other ions tested (Mn2+, Cu2+, Zn2+) do not interfere with the assay, presumably no other ionic species are present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni2+ in a variety of samples, which gives a linear range of 2 to 50 nmol nickel containing less than 25 nmol cobalt.

This product is manufactured by BioVision, an Abcam company and was previously called K510 Nickel Colorimetric Assay Kit. K510-100 is the same size as the 100 test size of ab241008.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye