In the Enolase Assay Kit (ab241024) enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with the probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm).
This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K691 Enolase Activity Colorimetric/Fluorometric Assay Kit. K691-100 is the same size as the 100 test size of ab241024.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.