abcam COX2 Inhibitor Screening Kit (Fluorometric)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

In COX2 Inhibitor Screening Kit (Fluorometric)protocol, the enzyme COX-2 generates Prostaglandin G2 (PGG2), an intermediate product in the COX pathway; the intensity of this signal decreases when a COX-2 inhibitor is present, allowing for the identification of potential COX-2 inhibiting compounds by comparing the fluorescence levels in the presence and absence of a test compound.

COX2 Inhibitor Screening Kit (Fluorometric) protocol summary:

- Add samples and standards to wells

- Add reaction mix

- Analyze with fluorescence microplate reader (Ex/Em = 535/587 nm)

This product is manufactured by BioVision, an Abcam company and was previously called K547 COX-2 Inhibitor Screening Kit (Fluorometric). K547-100 is the same size as the 100 test size of ab283401.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye