abcam Cathepsin B Activity Assay Kit (Fluorometric)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

How the assay works

Cathepsin B Activity Assay Kit (Fluorometric) utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin).

Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate RR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader at Ex/Em 400/505 nm.

Cathepsin B Activity Assay Kit protocol summary:

- Prepare samples

- Add Cathepsin B Reaction Buffer/reaction buffer

- Add Ac-RR-AFC substrate

- Incubate for 1 -2 hours at 37°C

- Measure fluorescence (Ex/Em = 400/505 nm)

Related and recommended products

See below other alternative Cathepsin Assay Kits:

- Cathepsin L Assay Kit (Magic Red) Cathepsin L Assay Kit (Magic Red)ab270774

- Cathepsin K Assay Kit (Magic Red) Cathepsin K Assay Kit (Magic Red)ab270773

This product is manufactured by BioVision, an Abcam company and was previously called K140 Cathepsin B Activity Fluorometric Assay Kit. K140-100 is the same size as the 100 test size of ab65300.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye