abcam Calpain Activity Assay Kit

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Please refer to protocols

Calpain Activity Assay Kit ab65308 provides optimized buffers and reagents for a convenient measurement of calpain activity.

The extraction buffer included in the kit specifically extracts cytosolic proteins without contamination by cell membrane and lysosome proteases. It also prevents auto-activation of calpain during the extraction procedure. Thus, the kit detects only activated calpain within the cytosol.

The calpain activity assay protocol is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by calpain, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence plate reader. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity.

Calpain activity assay protocol summary:

- add samples and positive and negative controls to wells

- add reaction buffer and calpain substrate

- incubate for 60 min

- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K240 Calpain Activity Fluorometric Assay Kit. K240-100 is the same size as the 100 test size of ab65308.

If additional Ac-LLY-AFC substrate is needed, it can be purchased separately as ab171379.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye