Total Antioxidant Capacity Assay Kit ab65329 can measure
either the combination of both small molecule antioxidants and proteins, or
small molecules alone in the presence of our proprietary Protein Mask.
In the total antioxidant capacity assay protocol, the Cu2+ ion
is converted to Cu+ by both small molecule and protein
antioxidants. The Protein Mask prevents Cu2+ reduction by
proteins, enabling the analysis of only the small molecule antioxidants. The
reduced Cu+ ion is chelated with a colorimetric probe giving a
broad absorbance peak around 570 nm, proportional to the total antioxidant
capacity.
Total antioxidant capacity assay protocol summary:
- add protein mask to samples if only measuring small molecule total
antioxidant capacity
- add samples and standards to wells
- add Cu2+ solution and incubate for 90 min at room temp
- analyze with microplate reader
Antioxidants play an important role in preventing the
formation of, and scavenging of, free radicals and other oxidizing species.
There are three categories of antioxidant species: enzyme systems (GSH
reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid,
GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). Trolox is used
to standardize antioxidants, with all other antioxidants being measured in
Trolox equivalents. Measurement of the combined non-enzymatic antioxidant capacity
of biological fluids and other samples provides an indication of the overall
capability to counteract reactive oxygen species (ROS), resist oxidative damage
and combat oxidative stress-related diseases.
Related antioxidant capacity assays and oxidative stress
products
The most popular antioxidant capacity assay is Total
Antioxidant Capacity Assay Kit ab65329, based on copper reduction. We also
supply FRAP Assay Kit FRAP
Assay Kit (Ferric Reducing Antioxidant Power Assay)ab234626 based on
iron reduction, DPPH Antioxidant Assay Kit DPPH
Antioxidant Assay Kit (Colorimetric)ab289847 based on reduction of the
DPPH probe, and ORAC Assay Kit ORAC
Assay Kitab233473.
Read about our full range of oxidative stress assays here.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
