Maltose and Glucose Assay Kit (ab65335) can measure glucose
or maltose levels in a variety of biological samples (e.g. serum, plasma, body
fluids, food, growth medium, etc.). The assay is based on the ability of
glucose oxidase to specifically oxidize free glucose, producing a product that
interacts with the glucose probe. The amount of product can be either
colorimetric (spectrophotometry at OD= 570 nm) or fluorometric (Ex/Em = 535/587
nm) method. This kit can detect 10 pmol to 10 nmol glucose per assay. It can
also be used for isomaltose detection. This product is manufactured by
BioVision, an Abcam company and was previously called K618 Maltose and Glucose
Colorimetric/Fluorometric Assay Kit. K618-100 is the same size as the 100 test
size of ab65335. Glucose (C H O ; FW: 180.16) and Maltose (C H O ; FW: 342.3)
are the main fuel sources to generate the universal energy molecule ATP.
Maltose is the major disaccharide that generated from hydrolysis of starch in
food. Maltose contains two glucose units joined by a α-1,4-glycosidic linkage,
which can be easily converted to two glucoses by α-D-glucosidase.