Triglyceride Assay Kit (ab65336) is a sensitive, easy assay
to measure triglyceride concentration in mammalian samples.
Other names used to refer to this type of assay include TAG
assay, TG assay, and triacylglycerol assay.
How the Triglyceride assay works
In the triglyceride assay protocol, triglycerides are converted to free fatty
acids and glycerol by a lipase enzyme. Glycerol is then phosphorylated by
glycerol kinase, and subsequently oxidized by glycerol phosphate oxidase to
produce hydrogen peroxide. The hydrogen peroxide reacts with a probe via a
peroxidase to generate color (spectrophotometry at λ= 570 nm) and fluorescence
(Ex/Em = 535/587 nm).
Triglyceride assay protocol summary
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Add samples and standards to wells.
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Add assay buffer and lipase, and incubate for 20 min.
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Add triglyceride reaction mix and incubate for 60 min.
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Analyze with microplate reader.
Getting the best performance from ab65336
Please note: the general range is 0-10 nmol (colorimetric) and 0-1 nmol
(fluorometric).
If your sample contains reducing substances, they are likely
to interfere with the assay. In this case, we recommend using Triglyceride
Assay Kit (Fluorometric, Reducing samples) Triglyceride
Assay Kit (Fluorometric, Reducing Samples)ab178780.
Technical Note: Triglyceride is unlikely to be detectable in
healthy urine but may be elevated in the presence of infection or disease of
the urinary tract.
How other researchers are using Triglyceride Assay Kit
ab65336
The Triglyceride assay kit has been used in publications in a variety of sample
types, including:
- -
Human: serum1, plasma2, mammary epithelial and
mammary cancer cell line lysate3, Huh7.5 hepatocyte-derived
cell line lysate4, primary liver cell line lysates5,
sebocyte cell culture lysates6
- -
Mouse: hepatocyte cell lysates7, liver extract8,
serum9, plasma10, kidney extracts11,
liver tissue and serum12, cardiac tissue extracts13
- -
Rat: liver tissue extract14, plasma15
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Drosophila16
References: 1-Huang Y et al. 2019, PMID: 30778327,
2-Wilson et al 2018; PMID: 29534545, 3-Yen MC et al. 2019, PMID: 30911270,
4-Kim D et al. 2018, PMID: 30127285, 5-Boteon Y et al. 2018, PMID: 30044872, 6-
Jin S and Lee MY 2018, PMID: 30400322, 7-Zhang W et al. 2019, PMID: 30664220,
8-Brial F et al. 2019, PMID: 30842494, 9-Liu J et al. 2018, PMID: 30344653 and
Patton A et al. 2018, PMID: 29666152, 10-Fernandes et al. 2018, PMID: 29320745
and Körholz JC et al 2018, PMID: 30362941 and Liang et al. 2018, PMID:
29659562, 11-Ding W et al. 2018, PMID: 29563333, 12-Cui XB et al. 2018, PMID:
29449334, 13-Rohm M et al. 2018<. PMID: 29610263, 14-Yu S et al. 2018, PMID:
29867579, 15-García-Ruiz et al. 2018, PMID: 29367725, 16-Wen CA and Ballard JWO
2019, PMID: 30805163
Related and recommended products
Triglyceride assay kit ab65336 is often used with Free Fatty Acid assay
kit Free
Fatty Acid Assay Kit - Quantificationab65341, and Cholesterol assay
kit Cholesterol
Assay Kit - HDL and LDL/VLDLab65390, to study lipid metabolism and its role
in various metabolic diseases such as diabetes, obesity, liver diseases, and
cardiovascular conditions.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously
called K622 Triglyceride Quantification Colorimetric/Fluorometric Kit. K622-100
is the same size as the 100 test size of ab65336.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
