Creatinine Assay Kit ab65340 provides an accurate,
convenient measure of creatinine concentration in biological fluids such as
serum, urine or CSF.
How the assay works
In the creatinine assay protocol, creatinine is converted to creatine by
creatininase, creatine is converted to sarcosine by creatinase. Sarcosine is
then oxidized by sarcosine oxidase producing hydrogen peroxide. Hydrogen
peroxide is then processed by a peroxidase causing a reaction with a probe to
generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm).
Creatinine assay protocol summary
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Add samples and standards to wells.
- -
Add reaction mix and incubate for 60 min at 37°C.
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Analyze with microplate reader.
Getting the best performance from ab65340
Unlike picric acid assays, this kit is suitable for serum creatinine
determinations, as well as for urine and other biological samples.
For deproteinization of samples: Better results are
typically seen with this assay when using a 10kda filter for sample
deproteinization than when using the PCA method.
Related and recommended products
Creatinine assay kit ab65340 is commonly used by researchers to measure kidney
function.
Kidney function marker assays include:
The albumin-creatinine ratio is often measured by using in
combination Creatine Assay Kit ab65340, and either Mouse Albumin ELISA
Kit Mouse
Albumin ELISA Kitab108792 or Human Albumin ELISA Kit Human
Albumin ELISA Kitab227933.
The urea-creatinine ratio is often measured by using in
combination Urea Assay Kit Urea
Assay Kitab83362 and Creatinine Assay Kit ab65340.
Albumin Creatinine Ratio Assay Kit Albumin
Creatinine Ratio Assay Kitab241018 is suitable for urine and serum,
and combines a fluorescent albumin assay with a colorimetric creatinine assay
in a convenient pack.
Creatinine assay methods
There are two established methods of creatinine assays:
- Jaffe assay
The Jaffe assay method is based on the reaction of creatinine with picric acid
at an alkaline pH. The creatinine‐picrate complex has an absorbance of 500 nm.
- Enzymatic creatinine assay
The standard enzymatic creatinine assay uses the method described above for
ab65340.
The Jaffe method is substantially simpler and cheaper than
enzymatic creatinine assays, however it suffers substantially from interference
by metabolites found in urine and serum.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously
called K625 Creatinine Colorimetric/Fluorometric Assay Kit. K625-100 is the
same size as the 100 test size of ab65340.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
