Free Fatty Acid Assay Kit ab65341 uses a convenient,
sensitive enzyme-based method for detecting long-chain free fatty acids in
various mammalian and other samples, such as serum, plasma and other body
fluids, food, growth media, etc.
Other names used to refer to this type of assay include NEFA
assay and FFA assay.
How the assay works
Free Fatty Acid Assay kit ab65341 measures non-esterified free fatty acids;
specifically C-8 (octanoate) and longer fatty acids.
In the free fatty acid assay protocol, free fatty acids are
converted to their coenzyme A derivatives by Acyl-CoA Synthetase. These are
then oxidized by Acyl CoA Oxidase in a reaction that produces hydrogen
peroxide. Hydrogen peroxide is then processed by an enzyme causing a reaction
with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em =
535/587 nm). Palmitic acid is used to generate a standard curve.
The free fatty acid assay method using Acyl-CoA Synthetase
and Acyl CoA Oxidase as used in ab65341 is the standard method used for free
fatty acid assays.
Free fatty acid assay protocol summary
- -
Add samples and standards to wells.
- -
Add ACS Reagent and incubate for 30 min at 37°C.
- -
Add reaction mix and incubate for 30 min at 37°C.
- -
Analyze with microplate reader.
How other researchers are using Free Fatty Acid Assay Kit
ab65341
This Free Fatty Acid assay kit has been used in publications in a variety of
sample types, including:
- -
Human: cell culture lysates1, serum2
- -
Mouse: plasma and liver tissue3, serum4, plasma5,
heart tissue6, liver7
- -
Rat: serum8, plasma9, cytosol of articular
chondrocyte primary cell cultures10
- -
Cell culture medium11
- - C
reinhardtii algae12
- -
Drosophila13
- - C
elegans14
References: 1 - Ali A et al 2018, Phokrai P et al 2018;
2 - Tahapary DL et al 2018; 3 - Becares et al 2019; 4 - Pan J et al 2019,
Maatta J et al 2018, Rohm M et al 2018, Shimazu T et al 2018; 5 - Woo M et al
2018, Tian X et al 2017; 6 - Liu W et al 2017; 7 - Hao et al 2017, Rui W et al
2016; 8 - Honore SM et al 2018; 9 - Van der Werf et al 2018;10 - Lee SW et al
2018; 11 - Kostrzewski T et al 2017; 12 - Ramanan R et al 2018; 13 - Scopelliti
A et al 2019; 14 - Pollard AK et al 2019
Related and recommended products
Free Fatty Acid assay kit ab65341is often used with Triglyceride assay
kit Triglyceride
Assay Kit - Quantificationab65336, and Cholesterol assay kit Cholesterol
Assay Kit - HDL and LDL/VLDLab65390, to study lipid metabolism and its role
in various metabolic diseases such as diabetes, obesity, liver diseases, and
cardiovascular conditions.
Other notes
This product is manufactured by BioVision, an Abcam company, and was previously
called K612 Free Fatty Acid Quantification Assay Kit.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
