Glycogen Assay Kit ab65620 is an easy and accurate assay to
measure glycogen levels in biological samples.
How the assay works
In the glycogen assay protocol, glucoamylase hydrolyzes the glycogen to glucose
which is then specifically oxidized to produce a product that reacts with
OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The
assay can detect glycogen 0.04 to 2 mg/ml.
Glycogen assay protocol summary
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Add samples and standards to wells
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Add hydrolysis enzyme mix and incubate for 30 min
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Add reaction mix and incubate for 30 min
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Analyze with microplate reader
Related Glycogen assay products
If your sample is likely to contain reducing substances, we recommend using
Glycogen Assay Kit II Glycogen
Assay Kit II (Colorimetric)ab169558, as reducing substances may interfere
with the assay detection method used with ab65620.
Glycogen
Assay Kit II (Colorimetric)ab169558 uses an alternative assay method,
where glucoamylase hydrolyzes glycogen to glucose, followed by an enzymatic
step which produces NADH, which is then used to reduce a tetrazolium dye,
producing a colored product.
If you are running a 384 well assay, we recommend Glycogen
Colorimetric Assay Kit Glycogen
Colorimetric Assay Kitab282931, which uses an identical assay method to
ab65620, and is formatted for 384 well use.
How other researchers are using Glycogen Assay Kit
ab65620
This glycogen assay kit has been used in publications in a variety of sample
types, including:
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Human: muscle tissue1
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Mouse: muscle tissue lysates2, muscle and liver tissue3,
liver4, cultured muscle myotubes5, astrocyte primary
cell lysates6,
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Rat: liver7, neuron-astrocyte co-cultures8
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Bacteria: M. buryatense9, Haemophilus influenzae10
References: 1 - Vaughan D et al 2016, Trewin AJ et al
2015; 2 - Baligand C et al 2017, Riedl et al 2016, Wicks SE et al 2015, Todd AG
et al 2015, Lundell LS et al 2019, Kim HY et al 2016, Amoasii et al 2016; 3 -
Xirouchaki CE et al 2016, Pamir N et al 2015, Zachwieja NJ et al 2016; 4 -
Pursell et al 2018; 5 - Park M et al 2016; 6 - Choudhury GR et al 2015; 7 -
Xiang L et al 2014, Guo J et al 2018; 8 - Sobieski C et al 2018; 9 - Puri AW et
al 2015; 10 - Wu S et al 2014
Glycogen assay methods
There are 3 major methods used to assay glycogen levels:
- -
Use of enzymes to produce glucose or glucose-1-phosphate from glycogen,
with production of a colored reaction product (Passonneau et al 1974 PMID
4844560), such as in ab65620.
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The anthrone method (Roe JH et al 1966, PMID 4289896) which uses alkaline
digestion of glycogen, precipitation of undigested proteins with excess
acid, and adds the anthrone reagent to detect glucose in a
spectrophotomer. The major disadvantage of the anthrone method is the use
of hazardous concentrated sulfuric acid which is heated in a >90°C
water bath.
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The phenol‐sulfuric acid method (described in Schaubroeck et al 2022, PMID
35179318), in which sulfuric acid dehydrates glycogen to
5‐hydroxymethylfurfural, which reacts with phenol to generate an
orange‐colored solution, the absorbance of which can be measured
spectrophotometrically. The major disadvantages of this method are the
requirement to handle and resuspend pellets after centrifugation which can
be fiddly, and the use of hazardous concentrated sulfuric acid and phenol.
Related and recommended products
Learn more about our tools for obesity research, including antibodies and ELISA
kits to adipogenesis and lipid metabolism markers, tools for GLP-1 receptor
agonist research, and enzymatic assays to key biochemicals and enzymes involved
in obesity-related metabolism.
Other notes
This product was previously called K646 BioVision Glycogen
Colorimetric/Fluorometric Assay Kit. Abcam acquired BioVision in 2021."
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
