abcam Ascorbic Acid Assay Kit (Biological Samples)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Ascorbic Acid Assay Kit (Biological Samples) (ab65656) provides a rapid, simple, and sensitive means of detecting ascorbic acid (vitamin C) in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products.

In the ascorbic acid assay protocol, Fe3+ is reduced to Fe2+ by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between OD=545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content.

The assay can detect 0.2 to 20 nmol of ascorbic acid.

Ascorbic acid assay protocol summary:

- add samples and standards to wells

- add reaction mix

- analyze with a microplate reader in kinetic mode every minute for 2-3 minutes

This product is manufactured by BioVision, an Abcam company and was previously called K671 Ascorbic Acid Colorimetric Assay Kit II (FRASC). K671-100 is the same size as the 100 test size of ab65656.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye