Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Urea Assay Kit ab83362 is a rapid, simple, sensitive, and
reliable assay used to quantify urea in a variety of samples such as serum,
plasma, and urine, etc. It can be used as a blood urea nitrogen assay kit (BUN
assay kit).
How the assay works
In the urea assay protocol, urea is acted on by enzymes to form a product that
reacts with a probe to generate color (ODmax=570nm). The absorbance is directly
proportional to the concentration of urea in the solution. The kit can detect
as low as 0.5 nmol per well or 10 μM of urea.
Urea assay protocol summary
- -
Add samples and standards to wells
- -
Add reaction mix and incubate for 60 min at 37°C
- -
Analyze with microplate reader
Urea assay methods
There are three urea assay methods to run blood urea nitrogen assays that are
commonly used in biological research:
- a)
Jung's method based on o-phthalaldehyde and N-(1-naphthyl)ethylenediamine,
in which o-phthalaldehyde reacts with urea, followed by a reaction with
N-(1-naphthyl)ethylenediamine, or an alternative chemical, to form a
colored product. Jung's method is simple, with the addition of a single
working reagent, with a short incubation at room temperature followed by
colorimetric readout. However the reagents include hazardous sulfuric
acid.
- Abcam
provides Urea Assay Kit Urea
Assay Kit IIab234052 which is based on an improved version of
Jung's method.
- b)
An enzymatic assay based on the conversion of urea to ammonium and carbon
dioxide by urease, followed by measurement of the ammonium level by
reaction with Berthelot's reagent (an alkaline solution of phenol and
hypochlorite) to form a colored product, or an alternative method of
ammonium readout
- c)
An enzymatic assay based on the conversion of urea to ammonium and carbon
dioxide by urease, followed by the conversion of ammonium and NADH to
glutamate and NAD+ by glutamate dehydrogenase. The assay can then either
readout by measuring the decrease in the 340 nm absorbance of NADH, or by
use of further enzymes to produce a colored product that increases
proportionate to the amount of urea in the sample.
- Urea
Assay Kit ab83362 is based on the glutamate dehydrogenase method, with
further enzymes, to allow a positive readout.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously
called K375 Urea Colorimetric Assay Kit. K375-100 is the same size as the 100
test size of ab83362.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
