abcam Branched Chain Amino Acid Assay Kit / BCAA Assay Kit

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Branched Chain Amino Acid Assay Kit / BCAA Assay Kit ab83374 provides a simple convenient means of measuring the BCAA's in biological samples.

The BCAA assay protocol uses an enzyme reaction in which BCAA is oxidatively deaminated, producing NADH which reduces a probe, generating a colored product (λmax = 450 nm).

The Branched Chain Amino Acid Assay Kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 μM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).

BCAA assay protocol summary:

- add samples and standards to wells

- add reaction mix and incubate for 30 min

- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K564 Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit. K564-100 is the same size as the 100 test size of ab83374.

The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye