Aconitase Assay Kit ab83459 is a highly sensitive, simple,
direct and HTS-ready colorimetric assay for measuring Aconitase activity in
biological samples.
In the aconitase assay protocol, citrate is converted by
aconitase into isocitrate, which is further processed resulting in a product
that converts a nearly colorless probe into an intensely colored form with a
max absorbance at 450nm.
Aconitase assay protocol summary:
- activate aconitase in samples by adding cysteine HCl and (NH4)2Fe(SO4)2
- add samples and standards to wells
- add reaction mix and incubate for 30-60 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company
and was previously called K716 Aconitase Activity Colorimetric Assay Kit.
K716-100 is the same size as the 100 test size of ab83459.
Aconitase (aconitate hydratase; EC 4.2.1.3) is an
iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the
stereospecific isomerization of citrate to isocitrate via cis-aconitate in the
tricarboxylic acid cycle, a non-redox-active process.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
