biotechrabbit™ Multiplex PCR Master Mix is a perfect choice for endpoint multiplex PCR. The unique buffer composition is optimized for robust simultaneous amplification of 10 or more targets from 50 bp – 2 kb in a single reaction.
The Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized Multiplex PCR buffer; thus, only template, PCR primers and PCR-grade water are added. The enhancers included in the mix enable efficient amplification of low abundant or GC-rich templates.
Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. The hot-start efficiently minimizes primer–dimers and mispriming.
