Excellent performance in transcription of complex RNA secondary structures
High yields of cDNA even with targets in low copy number
RNase H activity specific to RNA hybridized to cDNA for improved 1step PCR
biotechrabbit™ AllScript Reverse Transcriptase is a proprietary RT designed for highly specific and sensitive reverse transcription. It guarantees top performance in standard reverse transcription, synthesis of ds cDNA for cloning, RT-PCR and qRT-PCR, rapid amplification of cDNA ends (RACE) or RNA analysis by primer extension. It’s high affinity to RNA allows transcription of complex RNA secondary structures and targets in low copy number, leading to high yields of cDNA.
AllScript Reverse Transcriptase is a multifunctional enzyme including RNA-dependent and ssDNA-dependent DNA polymerase, as well as RNase H activity. The RNase H activity is specific to RNA hybridized to cDNA, with no effect on pure RNA template, resulting in improved performance of subsequent PCR.
Standard reverse transcription
Synthesis of ds cDNA for cloning
RT-PCR and qRT-PCR
Rapid amplification of cDNA ends (RACE)
RNA analysis by primer extension
QPS-101T
THUNDERBIRD® Probe qPCR Mix 1 ml × 1
50x ROX reference dye 50 µl × 1
QPS-101
THUNDERBIRD® Probe qPCR Mix 1.67 ml × 3
50x ROX reference dye 250 µl × 1
QPS-101X5
THUNDERBIRD® Probe qPCR Mix (1.67 ml × 3) x 5
50x ROX reference dye 250 µl × 5
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
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