biotechrabbit™ AnyScript Reverse Transcriptase is an improved and genetically engineered MMuLV Reverse Transcriptase with reduced RNase H activity and increased thermostability. The enzyme is ultrapure and free of RNases and Nucleases known to spoil RT reactions; this makes AnyScript a perfect choice for first strand cDNA synthesis at higher temperature that MMuLV. AnyScript is active up to 55 °C with high yield and full-length cDNA product. The enzyme is tailored to be employed in both two step and one step RT-PCR and qRT_PCR with high sensitivity and specificity.
Superb sensitivity and specificity make AnyScript an ideal enzyme for fast RT reaction from low template input in diagnostics application.
First-strand cDNA synthesis
cDNA library construction
RT-PCR
QPS-101T
THUNDERBIRD® Probe qPCR Mix 1 ml × 1
50x ROX reference dye 50 µl × 1
QPS-101
THUNDERBIRD® Probe qPCR Mix 1.67 ml × 3
50x ROX reference dye 250 µl × 1
QPS-101X5
THUNDERBIRD® Probe qPCR Mix (1.67 ml × 3) x 5
50x ROX reference dye 250 µl × 5
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
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