The biotechrabbit RTS 500 ProteoMaster E. coli HY Kit is designed and optimized for the expression of up to 6 mg of protein from plasmid templates in highly efficient coupled transcription/translation reactions. This is achieved by utilizing a biochemically enhanced high-yield (HY) E. coli lysate and the RTS 500 CECF reaction device.
Due to preparative expression yields, the RTS 500 Proteo-Master E. coli HY Kit is suited for the production of protein for NMR spectroscopy or X-ray crystallography. The kit design allows to use amino-acid mixtures of your choice. Methionine (included in the kit separately from other amino acids) can be easily substituted by selenomethionine (not included). Other amino acids can be exchanged by preparing tailored amino-acid mixtures using the RTS Amino Acid Sampler.
The RTS 500 ProteoMaster E. coli HY Kit is optimized for use in a thermomixer. The continuous agitation of the device guarantees optimal support of the CECF process, which is required for efficient protein expression.
(former product codes: RiNA N-1203-50, 5PRIME 2401500)
