The biotechrabbit RTS 500 E. coli Disulfide Kit provides an optimized combination of lysate, reagents, chaperones, and buffer systems to produce maximum yields of active proteins containing multiple disulfidebonds in a CECF system.
The kit employs several innovative technologies:
- Optimized high-yield (HY) E. coli lysate
- Redox buffer to maintain the system under oxidizing conditions, which enhances disulfide-bond formation
- Disulfide isomerase for rearrangement of disulfide bonds, which enhances the yields of correctly folded proteins
The CECF technology prolongs the reaction time up to 24 hours and, therefore, enhances protein yield. Transcription, translation and disulfide-bond formation take place in the reaction compartment of the device. Substrates and energy components needed for a sustained reaction are continuously supplied via a semipermeable membrane. At the same time, potentially inhibitory interactions with byproducts are diluted by diffusion through the same membrane into the 1 ml feeding chamber. Depending on the particular protein, expression of up to several milligrams of protein per reaction can be obtained (e.g., >2 mg/ml of active urokinase).
(former product codes: RiNA N-1403-50, 5PRIME 2401520)
