biotechrabbit RTS GroE Supplement

The Rapid Translation System (RTS) is an “open” cell-free protein expression system, in which reaction conditions can easily be adapted in a protein-specific manner by adding co-factors, ligands, detergents, or chaperones to the reaction mixture.

The biotechrabbit RTS GroE Supplement provides the GroEL and GroES (together referred to as GroE), which are cytoplasmic chaperones of E. coli reported to be involved in the de novo folding of 10–30 % of all cytoplasmic proteins. It is not possible to predict that a particular protein is a substrate for GroE, GroEL or GroES.

However, the presence of GroE during cellular or cell-free protein synthesis has been reported to lead to higher amounts of correctly folded and active product in many cases. The RTS GroE Supplement can be used with the RTS E. coli HY platform.

(former product codes: RiNA N-1602-05, 5PRIME 2401030)